Journal: Vaccine

Article Title: IFITM3-deficient mice as a model for testing influenza virus vaccines via the intramuscular route

doi: 10.1016/j.vaccine.2025.127458

Figure Lengend Snippet: A-D Lung viral titer measurements from WT or IFITM3 KO mice sacrificed 5 days p.i. with ( A ) 10 4 TCID50 of A/Victoria/361/2011 (H3N2) (WT n = 6, KO n = 6), ( B ) 10 4 TCID50 of B/Brisbane/33/2008 (Victoria lineage) (WT n = 6, KO n = 6), ( C ) 10 4 TCID50 of A/American Green-Winged Teal/Ohio/17OS1834/2017 (H10N8) (WT n = 3, KO n = 3), ( D ) 10 4 TCID50 of A/Black Duck/Tennessee/17OS036/2017 (H5N1) (WT n = 6, KO n = 6), ( E ) 10 5 TCID50 of A/Northern Shoveler/Mississippi/11OS145/2011 (H7N9) (WT n = 5, KO n = 5), and ( F ) 10 6 TCID50 of D/swine/OH/18TOSU0287/2018 (WT n = 8, KO n = 8). Error bars represent SEM, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by unpaired t-test.

Article Snippet: ELISA plates were coated overnight with 0.5 μg/mL of HA protein from A/Victoria/361/2011 (H3N2) (Sino Biological, Cat 40145-V08H1) in PBS.

Techniques: Northern Blot

Journal: Vaccine

Article Title: IFITM3-deficient mice as a model for testing influenza virus vaccines via the intramuscular route

doi: 10.1016/j.vaccine.2025.127458

Figure Lengend Snippet: A Schematic of the HA-based IAV prime-boost vaccination strategy for WT and IFITM3 KO mice using HA protein immunogen from strain A/Victoria/361/2011 (H3N2) (color scheme applies to B-F ). B-F Relative amounts of indicated IgG antibody in WT and IFITM3 KO mouse serum in vaccinated and mock-vaccinated animals measured by colorimetric ELISA measured at 450 nm (error bars represent SEM, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA followed by Tukey’s multiple comparisons test).

Article Snippet: ELISA plates were coated overnight with 0.5 μg/mL of HA protein from A/Victoria/361/2011 (H3N2) (Sino Biological, Cat 40145-V08H1) in PBS.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Vaccine

Article Title: IFITM3-deficient mice as a model for testing influenza virus vaccines via the intramuscular route

doi: 10.1016/j.vaccine.2025.127458

Figure Lengend Snippet: A Schematic of the HA-based IAV prime-boost vaccination strategy for WT and IFITM3 KO mice using HA protein immunogen from strain A/Victoria/361/2011 (H3N2) (color scheme applies to B-E ). B Micro-neutralization assay was performed with serum from boost-vaccinated and mock-vaccinated mice tested against A/Victoria/361/2011 (H3N2) (WT Mock n = 5, Vax n = 10; KO Mock n = 5, Vax n = 10; error bars indicate SEM; **p<0.01, ***p<0.001 by two-way ANOVA followed by Tukey’s multiple comparisons test). C Lung viral titers from boost-vaccinated and mock-vaccinated mice infected with 10,000 TCID50 A/Victoria/361/2011 (H3N2) (WT Mock n = 5, Vax n = 5; KO Mock n = 5, Vax n = 5; error bars represent SEM; ***p<0.001 by two-way ANOVA followed by Tukey’s multiple comparisons test). D Micro-neutralization assay was performed with serum from boost-vaccinated and mock-vaccinated mice tested against A/American Green-Winged Teal/Ohio/17OS1834/2017 (H10N8) (WT Mock n = 5, Vax n = 10; KO Mock n = 5, Vax n = 10). E Lung viral titers from boost-vaccinated and mock-vaccinated mice infected with 10,000 TCID50 A/American Green-Winged Teal/Ohio/17OS1834/2017 (H10N8) (WT Mock n = 3, Vax n = 5; KO Mock n = 3, Vax n = 5; error bars indicate SEM; p>0.05 by two-way ANOVA followed by Tukey’s multiple comparisons test).

Article Snippet: ELISA plates were coated overnight with 0.5 μg/mL of HA protein from A/Victoria/361/2011 (H3N2) (Sino Biological, Cat 40145-V08H1) in PBS.

Techniques: Neutralization, Infection

Journal: Vaccine

Article Title: IFITM3-deficient mice as a model for testing influenza virus vaccines via the intramuscular route

doi: 10.1016/j.vaccine.2025.127458

Figure Lengend Snippet: A-D Lung viral titer measurements from WT or IFITM3 KO mice sacrificed 5 days p.i. with ( A ) 10 4 TCID50 of A/Victoria/361/2011 (H3N2) (WT n = 6, KO n = 6), ( B ) 10 4 TCID50 of B/Brisbane/33/2008 (Victoria lineage) (WT n = 6, KO n = 6), ( C ) 10 4 TCID50 of A/American Green-Winged Teal/Ohio/17OS1834/2017 (H10N8) (WT n = 3, KO n = 3), ( D ) 10 4 TCID50 of A/Black Duck/Tennessee/17OS036/2017 (H5N1) (WT n = 6, KO n = 6), ( E ) 10 5 TCID50 of A/Northern Shoveler/Mississippi/11OS145/2011 (H7N9) (WT n = 5, KO n = 5), and ( F ) 10 6 TCID50 of D/swine/OH/18TOSU0287/2018 (WT n = 8, KO n = 8). Error bars represent SEM, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by unpaired t-test.

Article Snippet: C57BL/6N WT control animals (Charles River Laboratories, Strain 027) and KO mice were given 2 μg of HA protein from Influenza A/Victoria/361/2011 (H3N2) (Sino Biological, Cat 40145-V08H1) via 50 μL intramuscular injections.

Techniques: Northern Blot

Journal: Vaccine

Article Title: IFITM3-deficient mice as a model for testing influenza virus vaccines via the intramuscular route

doi: 10.1016/j.vaccine.2025.127458

Figure Lengend Snippet: A Schematic of the HA-based IAV prime-boost vaccination strategy for WT and IFITM3 KO mice using HA protein immunogen from strain A/Victoria/361/2011 (H3N2) (color scheme applies to B-F ). B-F Relative amounts of indicated IgG antibody in WT and IFITM3 KO mouse serum in vaccinated and mock-vaccinated animals measured by colorimetric ELISA measured at 450 nm (error bars represent SEM, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA followed by Tukey’s multiple comparisons test).

Article Snippet: C57BL/6N WT control animals (Charles River Laboratories, Strain 027) and KO mice were given 2 μg of HA protein from Influenza A/Victoria/361/2011 (H3N2) (Sino Biological, Cat 40145-V08H1) via 50 μL intramuscular injections.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Vaccine

Article Title: IFITM3-deficient mice as a model for testing influenza virus vaccines via the intramuscular route

doi: 10.1016/j.vaccine.2025.127458

Figure Lengend Snippet: A Schematic of the HA-based IAV prime-boost vaccination strategy for WT and IFITM3 KO mice using HA protein immunogen from strain A/Victoria/361/2011 (H3N2) (color scheme applies to B-E ). B Micro-neutralization assay was performed with serum from boost-vaccinated and mock-vaccinated mice tested against A/Victoria/361/2011 (H3N2) (WT Mock n = 5, Vax n = 10; KO Mock n = 5, Vax n = 10; error bars indicate SEM; **p<0.01, ***p<0.001 by two-way ANOVA followed by Tukey’s multiple comparisons test). C Lung viral titers from boost-vaccinated and mock-vaccinated mice infected with 10,000 TCID50 A/Victoria/361/2011 (H3N2) (WT Mock n = 5, Vax n = 5; KO Mock n = 5, Vax n = 5; error bars represent SEM; ***p<0.001 by two-way ANOVA followed by Tukey’s multiple comparisons test). D Micro-neutralization assay was performed with serum from boost-vaccinated and mock-vaccinated mice tested against A/American Green-Winged Teal/Ohio/17OS1834/2017 (H10N8) (WT Mock n = 5, Vax n = 10; KO Mock n = 5, Vax n = 10). E Lung viral titers from boost-vaccinated and mock-vaccinated mice infected with 10,000 TCID50 A/American Green-Winged Teal/Ohio/17OS1834/2017 (H10N8) (WT Mock n = 3, Vax n = 5; KO Mock n = 3, Vax n = 5; error bars indicate SEM; p>0.05 by two-way ANOVA followed by Tukey’s multiple comparisons test).

Article Snippet: C57BL/6N WT control animals (Charles River Laboratories, Strain 027) and KO mice were given 2 μg of HA protein from Influenza A/Victoria/361/2011 (H3N2) (Sino Biological, Cat 40145-V08H1) via 50 μL intramuscular injections.

Techniques: Neutralization, Infection